In this first discussion, you will learn two important techniques commonly used in the microbiology lab. Proceed to the
Virtual Interactive Bacteriology Laboratory
and complete the “Gram Stain” and “Streak Plate” exercises. Click on the pdf that accompanies each activity, and then select “Open Module” to complete the exercises and become more familiar with how we culture and identify bacteria in the laboratory. Within the module you will read the “Description,” and then the “Steps,” before you select “Start,” all from the main menu of the module. Keep your pdf open as you work through the exercises, so that you can refer back to the instructions. Once you have completed the exercises, respond to the following questions:
1) Why is it necessary to flame the inoculating loop each time you use it?
2) What part of the bacteria is “stained” by the Gram stain reagents?
3) What is Agar, and why is it used?
4) When “streaking” the bacterial culture onto the Agar plate, how does each successive “streak” establish a “pure culture”?
In this second Discussion, return to the Virtual Interactive Bacteriology Laboratory website, and complete the “Differential Media” lab exercises to prepare for your Case assignment, and participate in this Module’s discussion. Remember to keep the pdf Module Instructions open as you work through the module media. Once you have completed the exercises, respond to the following questions:
1) What is the difference between “Selective Media” and “Differential Media?
2) Why is blood used in the “Hemolytic Reactions”? How is this useful when identifying bacteria?
3) How will this module help you with your assignments in the “VUMIE” laboratory program?
For our third discussion, return to the Virtual Interactive Bacteriology Laboratory . Complete the first four biochemical tests: Catalase test, Coagulase Test, Oxidase Test, and Rapid Strep A test (remember to keep a copy of the pdf instructions open as you work through each test). Once you have completed these tests, briefly summarize what you learned from each test, and the significance of the test reagent to the identification of the bacteria. For example, what were you able to differentiate, and how? HINT: The “Description” section of each module will help you answer this question.
For your second post, complete the “Antimicrobial Susceptibility” module. Report your findings in your post. Use the “R,” “I,” “S” interpretation for each bacterial isolate.